Structure of genes for Hsp30 from the white-rot fungus Coriolus versicolor and the increase of their expression by heat shock and exposure to a hazardous chemical.

نویسندگان

  • Yosuke Iimura
  • Kenji Tatsumi
چکیده

We isolated and analysed two genomic DNAs that encode the heat-shock protein Hsp30 from Coriolus versicolor. The amino acid sequences substitute only three amino acid substitutions. The promoter regions contain the consensus heat-shock element, a xenobiotic-response element, a stress-response element, and a metal-response element. The levels of mRNAs for Hsp30 increased markedly after exposure of C. versicolor to pentachlorophenol and levels were higher than those after heat shock.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Bioremediation and Detoxification of the Textile Wastewater with Membrane Bioreactor Using the White-rot Fungus and Reuse of Wastewater

Background: Application of membrane technology to wastewater treatment has expanded over the last decades due to increasingly stringent legislation, greater opportunities for water reuse/recycling processes and continuing advancement in membrane technology. Objectives: In the present study, a bench-scale submerged microfiltration membrane bioreactor (MBR) was used to assess the treatment of tex...

متن کامل

Effect of enviromental temperature on heat shock proteins (HSP30, HSP70, HSP90) and IGF-I mRNA expression in Sparus aurata

Ambient temperature is one of the most important environmental factors affecting physiological mechanisms and biochemical reactions of living organisms. Thus the effect of ambient temperature on HSPs and IGF-I gene expression levels in the liver and muscle tissues of Sparus aurata were investigated in this research. The levels of HSPs, and IGF-I gene expression of the liver and muscle of Sparus...

متن کامل

Alternations of heat shock proteins (hsp70) gene expression in liver and gill of Persian sturgeon (Acipenser persicus Borodin, 1987) exposed to cadmium chloride

Induction of heat shock proteins (hsps) is considered as an important protective, ecophysiologically adaptive, and genetically conserved response to environmental stress in all organisms. The effects of exposure to sublethal doses of CdCl2 (0.05, 0.1 and 0.2 LC50) during 14 days on mRNA-hsp70 expression in liver and gillwere investigated in juveniles of Persian sturgeon (Acipenser persicus). Fi...

متن کامل

Biological Removal of Dibenzothiophene from Soil and Changes to soil Sulfate by White-Rot Fungus Phanerochaete chrysosporium

This study investigated biodegradation of dibenzothiophene (DBT) in marsh soil spiked bywhite-rot fungus Phanerochaete chrysosporium. Soil samples were spiked with 100 ppm DBTand incubated at 30°C in a dark chamber for 30 days. Samples were evaluated for pH, Mnperoxidaseactivity, sulfate ion concentration and growth during the tests. Results showedmaximum levels of pH, Mn-peroxidase and sulfate...

متن کامل

Polysaccharopeptides of Coriolus versicolor: physiological activity, uses, and production.

The protein-bound polysaccharides or polysaccharopeptides produced by Coriolus versicolor are effective immunopotentiators, which are used to supplement the chemotherapy and radiotherapy of cancers and various infectious diseases. Antitumor activity of polysaccharopeptides has been documented. Several kinds of protein-bound polysaccharides have been shown to be produced by the white rot fungus,...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Bioscience, biotechnology, and biochemistry

دوره 66 7  شماره 

صفحات  -

تاریخ انتشار 2002